Low Input Omics
In many experiments, only small quantities of biological material can be obtained, for instance from flow sorted cells or from embryonic tissues. Other samples such as those obtained from tumors are of heterogeneous composition, and ideally single cells should be analyzed in order to obtain biologically meaningful data. Down-scaling of standard protocols for sample preparation is not possible, as seriously biased data will result. Within Core for Life, protocols therefore are setup and benchmarked among participating institutions that allow robust interrogation of transcriptomes and genomes from samples consisting of few or single cells, in order to eliminate biases as much as possible.